You have full access to this article via your institution. So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

PCR-based techniques have transformed how scientists analyze transcripts’ translational applications in cancer and neuroscience research.

With the global overuse of antibiotics, the emergence of multidrug-resistant (MDR) bacteria and "superbugs" has become a major threat to human health and food safety. Among these, drug-resistant ...

Sample volumes that are gradually increasing and innovative options for molecular biology experiments are altering the demands on quantitative real-time PCR. Migrating to the multi-well format with ...

Early molecular response in patients (pts) with chronic myeloid leukemia in chronic phase (CML-CP) treated with dasatinib (DAS) or imatinib (IM) from DASISION. Survival outcomes of sustained MR4.5 in ...

Scientists around the world employ quantitative real-time PCR (qPCR) to measure the amount of particular nucleic acid sequences within a sample. While this technique is rapid, sensitive, and ...

A randomized trial of chemoradiation using mitomycin or cisplatin, with or without maintenance cisplatin/5FU in squamous cell carcinoma of the anus (ACT II) This is an ASCO Meeting Abstract from the ...

Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics. As a sensitive and precise method for quantitating specific nucleic acid targets, qPCR is ...