The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

Molecular biology has transitioned to a new era where precise genetic manipulation plays a critical role in research, medicine, and biotechnology. Among the vital tools in this field are cloning ...

The use of restriction enzymes to characterize DNA has been popular since the 1970s. Today, this technique remains one of the easiest and fastest ways to evaluate DNA sequences. As with most ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...